12/29/2023 0 Comments Snapgene codon optimizationSee Developability Analytics Assay DevelopmentĪTUM has extensive in-house capabilities including bio-layer interferometry for titer and affinity measurements using a pair of ForteBio Octets, aggregation detection by size exclusion chromatography, melting temperature and antibody stability measured using qPCR machines, glycan analysis by HPLC, and molecular mass determination using mass spectroscopy. We can design the full analytical package tailored to your molecule and subsequent applications including IND filing and GLP toxicology studies. Antibody AnalyticsĪTUM has a range of analytical services to characterize the identity, affinity, purity, quantity and quality of your antibody. We apply our GeneGPS® and VectorGPS® design platforms to optimize expression and balance chain ratios, maximizing the yield of your functional antibody. Variable regions can be combined with the original constant regions, or with new constant regions to generate humanized or isotype-switched antibodies. In addition, genome-wide correlations between protein levels and codon usage have been observed ( 12, 13 ). Whatever your discovery platform, we can take the sequence of the variable regions and turn them into real molecules. Codon optimization has long been used to enhance heterologous gene expression, and highly expressed proteins are mostly encoded by genes with strong codon biases for optimal codons. We clone and sequence independent clones to get you the sequence of the variable region, native secretion signal and the antibody isotype subclass. Antibody sequence information is essential for monoclonal antibody (mab) production, engineering and humanization, function optimization, database banking and patent applications. We'll prepare mRNA and make cDNA corresponding to the heavy and light chain by RACE. Learn More 2 Visualize Your Process Cloning is easier when you can see what you are doing. Test complicated projects, catch errors before they happen, and obtain the right constructs the first time. In-silico simulation of immune response using the vaccine as an antigen to generate a robust immune response. What’s New in SnapGene 6.1 Coronavirus Resources 1 Design Better Procedures Accurately design and simulate cloning procedures. It can significate increase protein expression level up to 50-fold, provided that the protein expression and purification methods are appropriately applied. Send us two tubes, each with a frozen pellet of ~500,000 hybridoma cells. Codon optimization and computational cloning of vaccine construct using JCat and SnapGene tool. GenScript OptimumGene algorithm provides a comprehensive solution strategy on optimizing all parameters that are critical to protein expression levels.
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